3A51
Structure of cytochrome P450 Vdh mutant (Vdh-K1) obtained by directed evolution with bound 25-hydroxyvitamin D3
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E | Vitamin D hydroxylase | polymer | 411 | 45587.9 | 5 | UniProt (C4B644) Pfam (PF00067) In PDB | Pseudonocardia autotrophica | |
2 | A, B, C, D, E | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 5 | Chemie (HEM) | |||
3 | E, A, B, C, D | 3-{2-[1-(5-HYDROXY-1,5-DIMETHYL-HEXYL)-7A-METHYL-OCTAHYDRO-INDEN-4-YLIDENE]-ETHYLIDENE}-4-METHYLENE-CYCLOHEXANOL | non-polymer | 400.6 | 5 | Chemie (VDY) | |||
4 | E, A, C, D | CALCIUM ION | non-polymer | 40.1 | 6 | Chemie (CA) | |||
5 | A | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
6 | E, A, B, C, D | ACETATE ION | non-polymer | 59.0 | 7 | Chemie (ACT) | |||
7 | water | water | 18.0 | 1319 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E: 1 - 403 (UniProt: C4B644)
PDB | External Database | Details |
---|---|---|
Arg 70 | Thr 70 | engineered mutation |
Leu 156 | Val 156 | engineered mutation |
Met 216 | Glu 216 | engineered mutation |
Arg 384 | Glu 384 | engineered mutation |
Leu 404 | - | expression tag |
Glu 405 | - | expression tag |
His 406 | - | expression tag |
His 407 | - | expression tag |
His 408 | - | expression tag |
His 409 | - | expression tag |
His 410 | - | expression tag |
His 411 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 227939.4 | |
Non-Polymers* | Number of molecules | 25 |
Total formula weight | 5923.6 | |
All* | Total formula weight | 233863.0 |