3A4X
Crystal structures of catalytic site mutants of active domain 2 of thermostable chitinase from Pyrococcus furiosus complexed with chito-oligosaccharides
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Chitinase | polymer | 311 | 34720.2 | 2 | UniProt (Q8U1H5) In PDB | Pyrococcus furiosus | |
| 2 | C, D | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose | branched | 830.8 | 2 | In PDB GlyTouCan (G93613RO) | |||
| 3 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 4 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 5 | A, B | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 6 | water | water | 18.0 | 641 | Chemie (HOH) |
Sequence modifications
A, B: 409 - 717 (UniProt: Q8U1H5)
| PDB | External Database | Details |
|---|---|---|
| Gly 407 | - | expression tag |
| Pro 408 | - | expression tag |
| Ala 524 | Asp 524 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 69440.4 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1661.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 605.1 | |
| All* | Total formula weight | 71707.1 |
