3A4X
Crystal structures of catalytic site mutants of active domain 2 of thermostable chitinase from Pyrococcus furiosus complexed with chito-oligosaccharides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Detector technology | CCD |
| Collection date | 2009-06-02 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.816, 91.943, 107.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.760 - 1.760 |
| R-factor | 0.15371 |
| Rwork | 0.153 |
| R-free | 0.17388 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dsk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.414 |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.760 |
| Number of reflections | 88323 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | VAPOR DIFFUSION, HANGING DROP, pH6.0, temperature 298K |
