35VB
Crystal structure of KRAS G13C (GMPPNP-bound) in complex with RAS-binding domain (RBD) and cysteine-rich domain (CRD) of BRAF
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Isoform 2B of GTPase KRas | polymer | 170 | 19374.9 | 1 | UniProt (P01116) Pfam (PF00071) | Homo sapiens (human) | K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras |
| 2 | B (B) | Serine/threonine-protein kinase B-raf | polymer | 135 | 15584.3 | 1 | UniProt (P15056) Pfam (PF02196) Pfam (PF00130) | Homo sapiens (human) | Proto-oncogene B-Raf,p94,v-Raf murine sarcoma viral oncogene homolog B1 |
| 3 | C (A) | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | non-polymer | 522.2 | 1 | Chemie (GNP) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 5 | E, F (B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) | |||
| 6 | G (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) PubChem (753) | |||
| 7 | H (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) PubChem (312) | |||
| 8 | I, J (A, B) | water | water | 18.0 | 12 | Chemie (HOH) |
Sequence modifications
A: 1 - 169 (UniProt: P01116)
B: 151 - 284 (UniProt: P15056)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Cys 13 | Gly 13 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 150 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 34959.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 804.9 | |
| All* | Total formula weight | 35764.0 |
