33CH

Cryo-EM structure of MAGE-A4 (230-239)-bound HLA-A*02:01 in complex with Fab VR-4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	MHC class I antigen	polymer	296	34155.8	1	UniProt (F6IQS1)	Homo sapiens (human)
2	B (B)	Beta-2-microglobulin	polymer	100	11879.4	1	UniProt (P61769)	Homo sapiens (human)
3	C (C)	Melanoma-associated antigen 4	polymer	10	1134.2	1	UniProt (P43358)	Homo sapiens	Cancer/testis antigen 1.4,CT1.4,MAGE-4 antigen,MAGE-41 antigen,MAGE-X2 antigen
4	D (F)	Fab BBM.1 light chain	polymer	214	23338.1	1		Mus musculus
5	E (G)	Fab BBM.1 heavy chain	polymer	222	23627.4	1		Mus musculus
6	F (D)	Fab VR-4 light chain	polymer	216	23747.3	1		Homo sapiens
7	G (E)	Fab VR-4 heavy chain	polymer	273	29592.9	1		Homo sapiens
8	H, I, J, K (A, C, D, E)	water	water		18.0	11	Chemie (HOH)

Sequence modifications

A: 1 - 278 (UniProt: F6IQS1)

PDB	External Database	Details
Met 0	-	initiating methionine
Cys 84	Tyr 108	conflict
Cys 139	Ala 163	conflict
Gly 277	Ser 301	conflict
Gly 279	-	expression tag
Ser 280	-	expression tag
Gly 281	-	expression tag
Leu 282	-	expression tag
Asn 283	-	expression tag
Asp 284	-	expression tag
Ile 285	-	expression tag
Phe 286	-	expression tag
Glu 287	-	expression tag
Ala 288	-	expression tag
Gln 289	-	expression tag
Lys 290	-	expression tag
Ile 291	-	expression tag
Glu 292	-	expression tag
Trp 293	-	expression tag
His 294	-	expression tag
Glu 295	-	expression tag

B: 1 - 99 (UniProt: P61769)

PDB	External Database	Details
Met 0	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	147474.9
All*	Total formula weight	147474.9

*Water molecules are not included.