32ZT

B. subtilis RNA Polymerase with SigF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (F)	RNA polymerase sigma-F factor	polymer	255	29415.5	1	UniProt (P07860)	Bacillus subtilis	Sporulation sigma factor,Stage II sporulation protein AC
2	B (O)	DNA-directed RNA polymerase subunit omega	polymer	67	7766.9	1	UniProt (O35011)	Bacillus subtilis	RNAP omega subunit,RNA polymerase omega 2 subunit,Transcriptase subunit omega
3	C (E)	DNA-directed RNA polymerase subunit epsilon	polymer	69	8228.4	1	UniProt (A0A9W5LJC9)	Bacillus subtilis	RNAP epsilon subunit,RNA polymerase epsilon subunit,Transcriptase subunit epsilon
4	D, H (V, U)	DNA-directed RNA polymerase subunit alpha	polymer	314	34842.4	2	UniProt (P20429)	Bacillus subtilis	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
5	E (Y)	DNA-directed RNA polymerase subunit beta'	polymer	1209	135826.4	1	UniProt (P37871)	Bacillus subtilis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
6	F (D)	DNA-directed RNA polymerase subunit delta	polymer	173	20417.9	1	UniProt (P12464)	Bacillus subtilis	RNAP delta factor
7	G (X)	DNA-directed RNA polymerase subunit beta	polymer	1193	133847.9	1	UniProt (P37870)	Bacillus subtilis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
8	I (K)	SigF controlled promoter PkatX	polymer	64	19682.6	1	GenBank (1169001324)	Bacillus subtilis
9	J (L)	SigF controlled promoter PkatX	polymer	64	19732.8	1	GenBank (1169001324)	Bacillus subtilis
10	K (Y)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
11	L, M (Y)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)

Sequence modifications

E: 1 - 69 (UniProt: A0A9W5LJC9)

PDB	External Database	Details
Leu 42	Phe 42	conflict

Y: 1 - 1199 (UniProt: P37871)

PDB	External Database	Details
His 1200	-	expression tag
His 1201	-	expression tag
His 1202	-	expression tag
His 1203	-	expression tag
His 1204	-	expression tag
His 1205	-	expression tag
His 1206	-	expression tag
His 1207	-	expression tag
His 1208	-	expression tag
His 1209	-	expression tag

K: 21 - 66 (GenBank: 1169001324)

PDB	External Database	Details
Dt 3	-	expression tag
Dt 4	-	expression tag
Dt 5	-	expression tag
Dt 6	-	expression tag
Dt 7	-	expression tag
Dt 8	-	expression tag
Dg 9	-	expression tag
Da 10	-	expression tag
Dt 11	-	expression tag
Dg 12	-	expression tag
Dc 13	-	expression tag
Dc 14	-	expression tag
Da 15	-	expression tag
Dt 16	-	expression tag
Dt 17	-	expression tag
Dg 18	-	expression tag
Dt 19	-	expression tag
Dt 20	-	expression tag
-	Dt 3838627	deletion

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	444603.1
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	444758.2

*Water molecules are not included.