31MR

CryoEM structure of a catalytically inactive CXC Chemokine-degrading protease SpyCEP from Streptococcus pyogenes complexed with an anti-PA-domain monoclonal antibody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cell envelope proteinase A	polymer	221	24609.8	1	UniProt (Q201Z7)	Streptococcus pyogenes	Peptidase S8 PrtS/CepA protein
2	B (B)	Cell envelope proteinase A	polymer	1346	148494.4	1	UniProt (Q201Z7)	Streptococcus pyogenes	Peptidase S8 PrtS/CepA protein
3	C (H)	Anti-PA-domain monoclonal antibody (3F2G10) Heavy chain variable region	polymer	122	13333.9	1		Mus musculus
4	D (L)	Anti-PA-domain monoclonal antibody (3F2G10) Light chain variable region	polymer	113	12324.8	1		Mus musculus

Sequence modifications

A: 2 - 212 (UniProt: Q201Z7)

PDB	External Database	Details
Met -8	-	initiating methionine
Gly -7	-	expression tag
Ser -6	-	expression tag
Ser -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Ala 119	Asp 151	engineered mutation

B: 213 - 1546 (UniProt: Q201Z7)

PDB	External Database	Details
Met 209	-	initiating methionine
Gly 210	-	expression tag
Ser 211	-	expression tag
Ser 212	-	expression tag
Ala 585	Ser 617	engineered mutation
Asn 884	Asp 916	variant
Leu 1547	-	expression tag
Glu 1548	-	expression tag
His 1549	-	expression tag
His 1550	-	expression tag
His 1551	-	expression tag
His 1552	-	expression tag
His 1553	-	expression tag
His 1554	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	198763.0
All*	Total formula weight	198763.0

*Water molecules are not included.