30XM
Engineered pyrrolysyl-tRNA synthetase (PylRS) from Methanosarcina barkeri (Mb) in complex with ATP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Pyrrolysine--tRNA ligase | polymer | 267 | 30792.3 | 2 | UniProt (Q6WRH6) | Methanosarcina barkeri | Pyrrolysine--tRNA(Pyl) ligase,Pyrrolysyl-tRNA synthetase,PylRS |
| 2 | C, H (A, B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 3 | D, E, F, I, J... (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 4 | G, L (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) PubChem (923) | |||
| 5 | M, N (A, B) | water | water | 18.0 | 301 | Chemie (HOH) |
Sequence modifications
A, B: 154 - 419 (UniProt: Q6WRH6)
| PDB | External Database | Details |
|---|---|---|
| Ser 153 | - | expression tag |
| Ala 271 | Tyr 271 | conflict |
| Gln 311 | Asn 311 | conflict |
| Ser 313 | Cys 313 | conflict |
| Trp 349 | Tyr 349 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 61584.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1206.2 | |
| All* | Total formula weight | 62790.8 |
