2ZZ1

Snapshot of the reaction from 6-CN-UMP to BMP catalyzed by Orotidine Monophosphate Deacarboxylase from M. thermoautotrophicum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Orotidine 5'-phosphate decarboxylase	polymer	252	27379.4	2	UniProt (O26232) Pfam (PF00215)	Methanothermobacter thermautotrophicus	OMP decarboxylase, OMPDCase, OMPdecase
2	C, H (A, B)	6-HYDROXYURIDINE-5'-PHOSPHATE	non-polymer		340.2	2	Chemie (BMP)
3	D, I (A, B)	6-cyanouridine 5'-phosphate	non-polymer		349.2	2	Chemie (6CN)
4	E, F, G, J (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	K, L (A, B)	water	water		18.0	287	Chemie (HOH)

Sequence modifications

A, B: 1 - 228 (UniProt: O26232)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Pro 101	Arg 101	SEE REMARK 999
Arg 226	Leu 226	engineered mutation
Ile 227	Asn 227	engineered mutation
Glu 229	-	expression tag
Asp 230	-	expression tag
Pro 231	-	expression tag
Ala 232	-	expression tag
Ala 233	-	expression tag
Asn 234	-	expression tag
Lys 235	-	expression tag
Ala 236	-	expression tag
Arg 237	-	expression tag
Lys 238	-	expression tag
Glu 239	-	expression tag
Ala 240	-	expression tag
Glu 241	-	expression tag
Leu 242	-	expression tag
Ala 243	-	expression tag
Ala 244	-	expression tag
Ala 245	-	expression tag
Thr 246	-	expression tag
Ala 247	-	expression tag
Glu 248	-	expression tag
Gln 249	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	54758.8
Non-Polymers*	Number of molecules	8
	Total formula weight	1747.1
All*	Total formula weight	56505.9

*Water molecules are not included.