2ZQB

Crystal structure of a psychrotrophic RNaseHI variant with sextuple thermostabilizing mutations

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Ribonuclease HI	polymer	158	17640.2	4	UniProt (Q8EE30) Pfam (PF00075) In PDB	Shewanella oneidensis	RNase HI
2	A, B, C	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
3		water	water		18.0	224	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 158 (UniProt: Q8EE30)

PDB	External Database	Details
Lys 29	Asn 29	engineered mutation
Gly 39	Asp 39	engineered mutation
Val 76	Met 76	engineered mutation
Asn 90	Lys 90	engineered mutation
Gly 97	Arg 97	engineered mutation
His 136	Asp 136	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	70560.7
Non-Polymers*	Number of molecules	3
	Total formula weight	288.2
All*	Total formula weight	70848.9

*Water molecules are not included.