2ZQB
Crystal structure of a psychrotrophic RNaseHI variant with sextuple thermostabilizing mutations
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Ribonuclease HI | polymer | 158 | 17640.2 | 4 | UniProt (Q8EE30) Pfam (PF00075) | Shewanella oneidensis | RNase HI |
| 2 | E, F, G (A, B, C) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 3 | H, I, J, K (A, B, C, D) | water | water | 18.0 | 224 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 158 (UniProt: Q8EE30)
| PDB | External Database | Details |
|---|---|---|
| Lys 29 | Asn 29 | engineered mutation |
| Gly 39 | Asp 39 | engineered mutation |
| Val 76 | Met 76 | engineered mutation |
| Asn 90 | Lys 90 | engineered mutation |
| Gly 97 | Arg 97 | engineered mutation |
| His 136 | Asp 136 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 70560.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 288.2 | |
| All* | Total formula weight | 70848.9 |
