2ZQB

Crystal structure of a psychrotrophic RNaseHI variant with sextuple thermostabilizing mutations

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003676	molecular_function	nucleic acid binding
A	0004519	molecular_function	endonuclease activity
A	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
A	0005737	cellular_component	cytoplasm
A	0006401	biological_process	RNA catabolic process
A	0043137	biological_process	DNA replication, removal of RNA primer
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0003676	molecular_function	nucleic acid binding
B	0004519	molecular_function	endonuclease activity
B	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
B	0005737	cellular_component	cytoplasm
B	0006401	biological_process	RNA catabolic process
B	0043137	biological_process	DNA replication, removal of RNA primer
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0003676	molecular_function	nucleic acid binding
C	0004519	molecular_function	endonuclease activity
C	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
C	0005737	cellular_component	cytoplasm
C	0006401	biological_process	RNA catabolic process
C	0043137	biological_process	DNA replication, removal of RNA primer
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0003676	molecular_function	nucleic acid binding
D	0004519	molecular_function	endonuclease activity
D	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
D	0005737	cellular_component	cytoplasm
D	0006401	biological_process	RNA catabolic process
D	0043137	biological_process	DNA replication, removal of RNA primer
D	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 159

Chain	Residue
A	THR45
A	ASN47
A	LYS101
A	ASN102
A	HOH215
C	ASN96

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site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 159

Chain	Residue
B	ASN102
B	HOH167
B	THR45
B	ASN47
B	LYS101

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site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 C 159

Chain	Residue
B	SER95
C	THR45
C	ASN47
C	LYS101
C	ASN102
C	HOH174

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00042

Chain	Residue	Details
A	ASP12
C	GLU50
C	ASP72
C	HIS136
D	ASP12
D	GLU50
D	ASP72
D	HIS136
A	GLU50
A	ASP72
A	HIS136
B	ASP12
B	GLU50
B	ASP72
B	HIS136
C	ASP12

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