2ZQ9
Cephalothin acyl-intermediate structure of class a beta-lactamase Toho-1 E166A/R274N/R276N triple mutant
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Beta-lactamase Toho-1 | polymer | 262 | 28117.8 | 1 | UniProt (Q47066) Pfam (PF00144) Pfam (PF00905) Pfam (PF13354) In PDB | Escherichia coli | |
2 | A | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
3 | A | 5-METHYLENE-2-[2-OXO-1-(2-THIOPHEN-2-YL-ACETYLAMINO)-ETHYL]-5,6-DIHYDRO-2H-[1,3]THIAZINE-4-CARBOXYLIC ACID | non-polymer | 338.4 | 1 | Chemie (CEO) | |||
4 | A | CEPHALOTHIN | non-polymer | 396.4 | 1 | Chemie (CLS) | |||
5 | A | THIOPHENEACETIC ACID | non-polymer | 142.2 | 1 | Chemie (SPA) | |||
6 | water | water | 18.0 | 353 | Chemie (HOH) |
Sequence modifications
A: 26 - 290 (UniProt: Q47066)
PDB | External Database | Details |
---|---|---|
Ala 166 | Glu 169 | engineered mutation |
Asn 274 | Arg 275 | engineered mutation |
Asn 276 | Arg 277 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 28117.8 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 1069.1 | |
All* | Total formula weight | 29186.9 |