2ZIW

Crystal structure of the Mus81-Eme1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mus81 protein	polymer	311	35333.6	1	UniProt (Q6GML8) Pfam (PF02732) Pfam (PF21292) UniProt (by SIFTS) (Q7SXA9)	Danio rerio (zebrafish)	DNA structure specific endonuclease Mus81
2	B (B)	Crossover junction endonuclease EME1	polymer	341	38282.2	1	UniProt (Q96AY2) Pfam (PF02732) Pfam (PF21292)	Homo sapiens (human)	hMMS4, DNA repair protein Eme1

Sequence modifications

A: 303 - 612 (UniProt: Q6GML8)

PDB	External Database	Details
Mse 302	-	initiating methionine

B: 246 - 570 (UniProt: Q96AY2)

PDB	External Database	Details
Mse 230	-	expression tag
Gly 231	-	expression tag
Ser 232	-	expression tag
Ser 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
Ser 240	-	expression tag
Gln 241	-	expression tag
Asp 242	-	expression tag
Pro 243	-	expression tag
Asn 244	-	expression tag
Ser 245	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73615.8
All*	Total formula weight	73615.8

*Water molecules are not included.