2ZIW
Crystal structure of the Mus81-Eme1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Detector technology | CCD |
Collection date | 2007-03-17 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 88.963, 88.963, 169.326 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.560 - 2.800 |
R-factor | 0.272 |
Rwork | 0.272 |
R-free | 0.32700 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.910 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | 0.275 |
Number of reflections | 21868 | |
<I/σ(I)> | 52.3 | 9.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.3 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.1M Na-Acetate, 2.3M ammonium chloride, VAPOR DIFFUSION, HANGING DROP, pH 5.0, temperature 277K |