2ZIR

Crystal Structure of rat protein farnesyltransferase complexed with a benzofuran inhibitor and FPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein farnesyltransferase subunit alpha	polymer	377	44086.1	1	UniProt (Q04631) Pfam (PF01239) In PDB	Rattus norvegicus (Rat)	CAAX farnesyltransferase subunit alpha, Ras proteins prenyltransferase alpha, FTase-alpha, Type I protein geranyl-geranyltransferase subunit alpha, GGTase-I-alpha
2	B	Protein farnesyltransferase subunit beta	polymer	440	49004.6	1	UniProt (Q02293) Pfam (PF00432) In PDB	Rattus norvegicus (Rat)	CAAX farnesyltransferase subunit beta, RAS proteins prenyltransferase beta, FTase-beta
3	A, B	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4	B	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	B	FARNESYL DIPHOSPHATE	non-polymer		382.3	1	Chemie (FPP)
6	B	2-[(S)-(4-chlorophenyl)(hydroxy)(1-methyl-1H-imidazol-5-yl)methyl]-N-morpholin-4-yl-7-phenyl-1-benzofuran-5-carboxamide	non-polymer		543.0	1	Chemie (NH7)
7		water	water		18.0	397	Chemie (HOH)

Sequence modifications

A: 1 - 377 (UniProt: Q04631)

PDB	External Database	Details
Thr 156	Ile 156	engineered mutation

B: 1 - 437 (UniProt: Q02293)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	93090.7
Non-Polymers*	Number of molecules	8
	Total formula weight	1451.2
All*	Total formula weight	94541.9

*Water molecules are not included.