2ZIM

Pyrrolysyl-tRNA synthetase bound to adenylated pyrrolysine and pyrophosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Pyrrolysyl-tRNA synthetase	polymer	291	33416.2	1	UniProt (Q8PWY1) Pfam (PF01409) In PDB	Methanosarcina mazei	Pyrrolysine--tRNA ligase, PylRS
2	A	1,2-ETHANEDIOL	non-polymer		62.1	10	Chemie (EDO)
3	A	(2R)-2-AMINO-6-({[(2S,3R)-3-METHYLPYRROLIDIN-2-YL]CARBONYL}AMINO)HEXANOYL [(2S,3R,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]METHYL HYDROGEN (R)-PHOSPHATE	non-polymer		586.5	1	Chemie (YLY)
4	A	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
5		water	water		18.0	186	Chemie (HOH)

Sequence modifications

A: 185 - 454 (UniProt: Q8PWY1)

PDB	External Database	Details
Met 164	-	expression tag
Gly 165	-	expression tag
Ser 166	-	expression tag
Ser 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
Ser 174	-	expression tag
Ser 175	-	expression tag
Gly 176	-	expression tag
Leu 177	-	expression tag
Val 178	-	expression tag
Pro 179	-	expression tag
Arg 180	-	expression tag
Gly 181	-	expression tag
Ser 182	-	expression tag
His 183	-	expression tag
Met 184	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33416.2
Non-Polymers*	Number of molecules	12
	Total formula weight	1383.2
All*	Total formula weight	34799.4

*Water molecules are not included.