2ZIM
Pyrrolysyl-tRNA synthetase bound to adenylated pyrrolysine and pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 64 |
| Unit cell lengths | 105.070, 105.070, 70.262 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.400 - 2.100 |
| R-factor | 0.18845 |
| Rwork | 0.186 |
| R-free | 0.22811 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.835 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.087 | |
| Number of reflections | 25910 | |
| <I/σ(I)> | 26.1 | |
| Completeness [%] | 100 | |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 289 | 100 MM TRIS, 10% PEG2000-MME, pH 7.5, VAPOR DIFFUSION, temperature 289 K |
