2ZET

Crystal structure of the small GTPase Rab27B complexed with the Slp homology domain of Slac2-a/melanophilin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ras-related protein Rab-27B	polymer	203	23235.3	2	UniProt (Q99P58) Pfam (PF00071) In PDB	Mus musculus (mouse)
2	C, D	Melanophilin	polymer	153	17722.2	2	UniProt (Q91V27) Pfam (PF02318) In PDB	Mus musculus (mouse)	Exophilin-3, Leaden protein, Synaptotagmin-like protein 2a, Slp homolog lacking C2 domains a, SlaC2-a
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, B	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
5	C, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
6	C, D	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7		water	water		18.0	9	Chemie (HOH)

Sequence modifications

A, B: 1 - 201 (UniProt: Q99P58)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Leu 78	Gln 78	engineered mutation

C, D: 1 - 146 (UniProt: Q91V27)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	81914.9
Non-Polymers*	Number of molecules	10
	Total formula weight	1548.7
All*	Total formula weight	83463.6

*Water molecules are not included.