2YPT
Crystal structure of the human nuclear membrane zinc metalloprotease ZMPSTE24 mutant (E336A) in complex with a synthetic CSIM tetrapeptide from the C-terminus of prelamin A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, D, E) | CAAX PRENYL PROTEASE 1 HOMOLOG | polymer | 482 | 55648.5 | 4 | UniProt (O75844) Pfam (PF16491) Pfam (PF01435) | HOMO SAPIENS (HUMAN) | FARNESYLATED PROTEINS-CONVERTING ENZYME 1, FACE-1, PRENYL PROTEIN-SPECIFIC ENDOPROTEASE 1, ZINC METALLOPROTEINASE STE24 HOMOLOG |
| 2 | E, F, G, H (F, G, H, I) | PRELAMIN-A/C | polymer | 4 | 452.6 | 4 | UniProt (P02545) | HOMO SAPIENS | 70 KDA LAMIN, RENAL CARCINOMA ANTIGEN NY-REN-32, PRELAMIN A |
| 3 | I, J, K, L (A, B, D, E) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
A, B, D, E: 1 - 475 (UniProt: O75844)
| PDB | External Database | Details |
|---|---|---|
| Ala 137 | Thr 137 | variant |
| Ala 336 | Glu 336 | engineered mutation |
| Ala 476 | - | expression tag |
| Glu 477 | - | expression tag |
| Asn 478 | - | expression tag |
| Leu 479 | - | expression tag |
| Tyr 480 | - | expression tag |
| Phe 481 | - | expression tag |
| Gln 482 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 224404.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 224665.9 |
