2YPT
Crystal structure of the human nuclear membrane zinc metalloprotease ZMPSTE24 mutant (E336A) in complex with a synthetic CSIM tetrapeptide from the C-terminus of prelamin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-26 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 61.320, 95.540, 132.030 |
Unit cell angles | 76.26, 79.67, 72.30 |
Refinement procedure
Resolution | 38.500 - 3.800 |
R-factor | 0.2635 |
Rwork | 0.263 |
R-free | 0.28110 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 0.860 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.500 | 3.900 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.050 | 0.690 |
Number of reflections | 26943 | |
<I/σ(I)> | 10.8 | 1.6 |
Completeness [%] | 99.2 | 99.4 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.1M HEPES PH 7.0, 0.1M CALCIUM CHLORIDE, 29%(V/V) PEG 400 |