2YPT
Crystal structure of the human nuclear membrane zinc metalloprotease ZMPSTE24 mutant (E336A) in complex with a synthetic CSIM tetrapeptide from the C-terminus of prelamin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.320, 95.540, 132.030 |
| Unit cell angles | 76.26, 79.67, 72.30 |
Refinement procedure
| Resolution | 38.500 - 3.800 |
| R-factor | 0.2635 |
| Rwork | 0.263 |
| R-free | 0.28110 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 3.900 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.050 | 0.690 |
| Number of reflections | 26943 | |
| <I/σ(I)> | 10.8 | 1.6 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1M HEPES PH 7.0, 0.1M CALCIUM CHLORIDE, 29%(V/V) PEG 400 |
