2YO0

Salmonella enterica SadA 1049-1304 fused to GCN4 adaptors (SadAK9-cfI)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	GENERAL CONTROL PROTEIN GCN4, PUTATIVE INNER MEMBRANE PROTEIN	polymer	322	34005.4	1	UniProt (P03069) UniProt (Q8ZL64) Pfam (PF05662) Pfam (PF05658) Pfam (PF07716)	SACCHAROMYCES CEREVISIAE	AMINO ACID BIOSYNTHESIS REGULATORY PROTEIN, AUTOTRANSPORTER ADHESIN FRAGMENT
2	B (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3	C (A)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A: 1020 - 1048 (UniProt: P03069)

PDB	External Database	Details
Ile 1023	Leu 253	engineered mutation
Ile 1027	Val 257	engineered mutation
Ile 1030	Leu 260	engineered mutation
Ile 1034	Asn 264	engineered mutation
Ile 1037	Leu 267	engineered mutation
Ile 1041	Val 271	engineered mutation
Ile 1044	Leu 274	engineered mutation
Ile 1048	Val 278	engineered mutation
Lys 1334	-	expression tag
Leu 1335	-	expression tag
His 1336	-	expression tag
His 1337	-	expression tag
His 1338	-	expression tag
His 1339	-	expression tag
His 1340	-	expression tag
His 1341	-	expression tag

A: 1305 - 1333 (UniProt: P03069)

PDB	External Database	Details
Ile 1308	Leu 253	engineered mutation
Ile 1312	Val 257	engineered mutation
Ile 1315	Leu 260	engineered mutation
Ile 1319	Asn 264	engineered mutation
Ile 1322	Leu 267	engineered mutation
Ile 1326	Val 271	engineered mutation
Ile 1329	Leu 274	engineered mutation
Ile 1333	Val 278	engineered mutation

A: 1049 - 1304 (UniProt: Q8ZL64)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34005.4
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	34040.9

*Water molecules are not included.