2YO0
Salmonella enterica SadA 1049-1304 fused to GCN4 adaptors (SadAK9-cfI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 54.000, 54.000, 306.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.530 - 2.800 |
| R-factor | 0.22469 |
| Rwork | 0.221 |
| R-free | 0.29738 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.140 | 0.600 |
| Number of reflections | 12334 | |
| <I/σ(I)> | 8.59 | 2.07 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 3.25 | 3.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% (V/V) BUTANEDIOL, 100 MM SODIUM ACETATE PH 4.5 |
