2YG7

Structure-based redesign of cofactor binding in Putrescine Oxidase: A394C-A396T-Q431G Triple mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PUTRESCINE OXIDASE	polymer	453	49388.0	2	UniProt (B0F9F6) Pfam (PF01593)	RHODOCOCCUS ERYTHROPOLIS
2	C, D (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	E, F (A, B)	water	water		18.0	40	Chemie (HOH)

Sequence modifications

A, B: 1 - 453 (UniProt: B0F9F6)

PDB	External Database	Details
Cys 394	Ala 394	engineered mutation
Thr 396	Ala 396	engineered mutation
Gly 431	Gln 431	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	98776.0
Non-Polymers*	Number of molecules	2
	Total formula weight	1571.1
All*	Total formula weight	100347.1

*Water molecules are not included.