2YG7
Structure-based redesign of cofactor binding in Putrescine Oxidase: A394C-A396T-Q431G Triple mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 114.010, 120.390, 171.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 98.520 - 2.750 |
| R-factor | 0.23262 |
| Rwork | 0.230 |
| R-free | 0.27626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yg3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.561 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.150 | 0.600 |
| Number of reflections | 31023 | |
| <I/σ(I)> | 7.7 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | pH 6.4 |
