2Y7S

Structure of a designed meningococcal antigen (factor H binding protein, mutant G1) inducing broad protective immunity

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	FACTOR H BINDING PROTEIN	polymer	256	27718.9	2	UniProt (Q9JXV4) Pfam (PF20937) Pfam (PF08794)	NEISSERIA MENINGITIDIS SEROGROUP B
2	C, D (A, B)	water	water		18.0	569	Chemie (HOH)

Sequence modifications

A, B: 8 - 254 (UniProt: Q9JXV4)

PDB	External Database	Details
Met 7	-	expression tag
Leu 255	-	expression tag
Glu 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
Leu 134	Ile 199	engineered mutation
Gly 135	Ala 200	engineered mutation
Ala 140	Ser 205	engineered mutation
Asn 142	Asp 207	engineered mutation
Gln 143	Lys 208	engineered mutation
Asp 146	Glu 211	engineered mutation
-	Gly 213	deletion
Lys 148	Arg 214	engineered mutation
Glu 150	Thr 216	engineered mutation
Thr 172	Ala 238	engineered mutation
Lys 173	Ala 239	engineered mutation
Glu 191	Asp 257	engineered mutation
Ser 194	Ala 260	engineered mutation
Glu 196	Asp 262	engineered mutation
Ala 199	Pro 265	engineered mutation
Ser 203	Arg 269	engineered mutation
Leu 208	Ser 274	engineered mutation
Asp 210	Ser 276	engineered mutation
Arg 212	Leu 278	engineered mutation
Gly 214	Asn 280	engineered mutation
Ser 215	Gln 281	engineered mutation
Glu 216	Ala 282	engineered mutation
Arg 229	Lys 295	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55437.8
All*	Total formula weight	55437.8

*Water molecules are not included.