2Y7Q

THE HIGH-AFFINITY COMPLEX BETWEEN IGE AND ITS RECEPTOR FC EPSILON RI

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	HIGH AFFINITY IMMUNOGLOBULIN EPSILON RECEPTOR SUBUNIT ALPHA	polymer	188	21722.1	1	UniProt (P12319) Pfam (PF13927) Pfam (PF13895)	HOMO SAPIENS (HUMAN)	HIGH AFFINITY IGE RECEPTOR FC EPSILON RI, IGE FC RECEPTOR SUBUNIT ALPHA, FC-EPSILON RI-ALPHA, FCERI
2	B, C (B, D)	IG EPSILON CHAIN C REGION	polymer	327	36354.8	2	UniProt (P01854) Pfam (PF07654)	HOMO SAPIENS (HUMAN)
3	D (C)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
4	E, F, G (A, B, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A: 1 - 176 (UniProt: P12319)

PDB	External Database	Details
Glu -2	-	cloning artifact
Thr -1	-	cloning artifact
Gly 0	-	cloning artifact
Ala 74	Asn 99	engineered mutation
Ala 135	Asn 160	engineered mutation
Ala 142	Thr 167	engineered mutation
Ala 143	Val 168	cloning artifact
Gly 177	-	cloning artifact
Thr 178	-	cloning artifact
Lys 179	-	cloning artifact
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag

B, D: 224 - 547 (UniProt: P01854)

PDB	External Database	Details
Asp 222	-	expression tag
Ile 223	-	expression tag
Ala 225	Cys 105	engineered mutation
Gln 265	Asn 146	engineered mutation
Gln 371	Asn 252	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	94431.7
Branched	Number of molecules	1
	Total formula weight	748.7
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	95844.0

*Water molecules are not included.