2Y6I

Crystal Structure of Collagenase G from Clostridium histolyticum in complex with Isoamylphosphonyl-Gly-Pro-Ala at 3.25 Angstrom Resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	COLLAGENASE	polymer	785	89031.9	1	UniProt (Q9X721) Pfam (PF08453) Pfam (PF01752) Pfam (PF18496) Pfam (PF18911)	CLOSTRIDIUM HISTOLYTICUM	COLLAGENASE G
2	B (B)	ISOAMYLPHOSPHONYL-GLY-PRO-ALA	polymer	4	377.4	1	BIRD (PRD_000989)	SYNTHETIC CONSTRUCT
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D (A)	CITRATE ANION	non-polymer		189.1	1	Chemie (FLC)
5	E, F, G (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	3	Chemie (TRS)
6	H, I (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
7	J (A)	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
8	K (A)	water	water		18.0	44	Chemie (HOH)

Sequence modifications

A: 119 - 880 (UniProt: Q9X721)

PDB	External Database	Details
Met 96	-	expression tag
Gly 97	-	expression tag
Ser 98	-	expression tag
Ser 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
His 105	-	expression tag
Ser 106	-	expression tag
Ser 107	-	expression tag
Gly 108	-	expression tag
Glu 109	-	expression tag
Asn 110	-	expression tag
Leu 111	-	expression tag
Tyr 112	-	expression tag
Phe 113	-	expression tag
Gln 114	-	expression tag
Gly 115	-	expression tag
Gly 116	-	expression tag
Thr 117	-	expression tag
Met 118	-	expression tag
Leu 575	Ser 575	SEE REMARK 999
Val 665	Ala 665	SEE REMARK 999
Gly 790	Ala 790	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	89409.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1071.5
All*	Total formula weight	90480.8

*Water molecules are not included.