2Y6I
Crystal Structure of Collagenase G from Clostridium histolyticum in complex with Isoamylphosphonyl-Gly-Pro-Ala at 3.25 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-12 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.120, 108.840, 181.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 93.280 - 3.250 |
R-factor | 0.21897 |
Rwork | 0.216 |
R-free | 0.26633 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.915 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.710 | 3.430 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.100 | 0.300 |
Number of reflections | 18719 | |
<I/σ(I)> | 11.7 | 4.7 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 6.5 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 21.5% PEG 3350, 0.225M TRISODIUMCITRATE, PH 8.3 |