2Y52

Crystal structure of E496A mutant of the box pathway encoded ALDH from Burkholderia xenovorans LB400

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ALDEHYDE DEHYDROGENASE (BOX PATHWAY)	polymer	534	55780.0	2	UniProt (Q13WK4) Pfam (PF00171)	BURKHOLDERIA XENOVORANS LB400	ALDEHYDE DEHYDROGENASE
2	C, D, E, F (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
3	G, H (A, B)	water	water		18.0	1332	Chemie (HOH)

Sequence modifications

A, B: 1 - 531 (UniProt: Q13WK4)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 496	Glu 496	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	111559.9
Non-Polymers*	Number of molecules	4
	Total formula weight	368.4
All*	Total formula weight	111928.3

*Water molecules are not included.