2Y52
Crystal structure of E496A mutant of the box pathway encoded ALDH from Burkholderia xenovorans LB400
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 58.050, 68.130, 78.080 |
Unit cell angles | 112.23, 90.56, 111.69 |
Refinement procedure
Resolution | 38.080 - 1.650 |
R-factor | 0.18298 |
Rwork | 0.180 |
R-free | 0.23107 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.027 |
RMSD bond angle | 2.002 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.080 |
High resolution limit [Å] | 1.650 |
Rmerge | 0.090 |
Number of reflections | 117024 |
<I/σ(I)> | 7.2 |
Completeness [%] | 95.9 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |