2XXB

Penta-mutant of Thermus thermophilus lactate dehydrogenase, complex with AMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	L-LACTATE DEHYDROGENASE	polymer	310	32599.4	2	UniProt (Q5SJA1) Pfam (PF00056) Pfam (PF02866) In PDB	THERMUS THERMOPHILUS	L-LDH
2	A, B	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
3		water	water		18.0	293	Chemie (HOH)

Sequence modifications

A, B: 22 - 331 (UniProt: Q5SJA1)

PDB	External Database	Details
Trp 79	Arg 58	engineered mutation
Ala 151	Arg 128	engineered mutation
Ala 279	Glu 260	engineered mutation
Ala 299	Glu 279	engineered mutation
Ala 313	Glu 292	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65198.7
Non-Polymers*	Number of molecules	2
	Total formula weight	694.4
All*	Total formula weight	65893.2

*Water molecules are not included.