2XXB

Penta-mutant of Thermus thermophilus lactate dehydrogenase, complex with AMP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
A	0005737	cellular_component	cytoplasm
A	0006089	biological_process	lactate metabolic process
A	0006096	biological_process	glycolytic process
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019752	biological_process	carboxylic acid metabolic process
B	0003824	molecular_function	catalytic activity
B	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
B	0005737	cellular_component	cytoplasm
B	0006089	biological_process	lactate metabolic process
B	0006096	biological_process	glycolytic process
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0019752	biological_process	carboxylic acid metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE AMP A 1332

Chain	Residue
A	VAL27
A	ALA98
A	GLY99
A	VAL119
A	VAL123
A	HOH2135
A	HOH2136
A	HOH2137
A	HOH2138
B	GLY106
B	GLN111
A	GLY28
A	GLY30
A	MET31
A	VAL32
A	ASP53
A	LEU54
A	LEU58
A	TYR85

---

site_id	AC2
Number of Residues	17
Details	BINDING SITE FOR RESIDUE AMP B 1332

Chain	Residue
B	GLY30
B	MET31
B	VAL32
B	ASP53
B	LEU54
B	LEU58
B	TYR85
B	ALA98
B	GLY99
B	VAL123
B	HOH2148
B	HOH2149
B	HOH2150
B	HOH2151
B	HOH2152
B	HOH2153
B	HOH2154

---

Functional Information from PROSITE/UniProt

site_id	PS00064
Number of Residues	7
Details	L_LDH L-lactate dehydrogenase active site. LGEHGDS

Chain	Residue	Details
A	LEU192-SER198

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17936781","evidenceCode":"ECO:0000305"},{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Lactate dehydrogenase from T. thermophilus, penta-mutant (ternary complex with AMP).","authors":["Diop F.","Coquelle N.","Tickle J.","De Mendoza Barbera E.","Vellieux F.M.D."]}},{"source":"PDB","id":"2V7P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XXJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17936781","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"NOV-2006","submissionDatabase":"PDB data bank","title":"Structure of TT0471 protein from Thermus thermophilus.","authors":["Lokanath N.K.","Kunishima N."]}},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Lactate dehydrogenase from T. thermophilus, penta-mutant (ternary complex with AMP).","authors":["Diop F.","Coquelle N.","Tickle J.","De Mendoza Barbera E.","Vellieux F.M.D."]}},{"source":"PubMed","id":"22319152","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2E37","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V7P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XXB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XXJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3ZZN","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17936781","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Lactate dehydrogenase from T. thermophilus, penta-mutant (ternary complex with AMP).","authors":["Diop F.","Coquelle N.","Tickle J.","De Mendoza Barbera E.","Vellieux F.M.D."]}},{"source":"PDB","id":"2V7P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XXB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XXJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17936781","evidenceCode":"ECO:0000305"},{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Lactate dehydrogenase from T. thermophilus, penta-mutant (ternary complex with AMP).","authors":["Diop F.","Coquelle N.","Tickle J.","De Mendoza Barbera E.","Vellieux F.M.D."]}},{"source":"PDB","id":"2V7P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XXJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17936781","evidenceCode":"ECO:0000305"},{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Lactate dehydrogenase from T. thermophilus, penta-mutant (ternary complex with AMP).","authors":["Diop F.","Coquelle N.","Tickle J.","De Mendoza Barbera E.","Vellieux F.M.D."]}},{"source":"PDB","id":"2V7P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XXJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17936781","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Lactate dehydrogenase from T. thermophilus, penta-mutant (ternary complex with AMP).","authors":["Diop F.","Coquelle N.","Tickle J.","De Mendoza Barbera E.","Vellieux F.M.D."]}},{"source":"PDB","id":"2V7P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XXJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17936781","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Lactate dehydrogenase from T. thermophilus, penta-mutant (ternary complex with AMP).","authors":["Diop F.","Coquelle N.","Tickle J.","De Mendoza Barbera E.","Vellieux F.M.D."]}},{"source":"PDB","id":"2V7P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XXJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"HAMAP-Rule","id":"MF_00488","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---