2XQ2

Structure of the K294A mutant of vSGLT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	SODIUM/GLUCOSE COTRANSPORTER	polymer	593	64142.3	1	UniProt (P96169) Pfam (PF00474)	VIBRIO PARAHAEMOLYTICUS	VSGLT, NA(+)/GLUCOSE SYMPORTER
2	B (B)	SODIUM/GLUCOSE COTRANSPORTER	polymer	593	62859.1	1	UniProt (P96169) Pfam (PF00474)	VIBRIO PARAHAEMOLYTICUS	VSGLT, NA(+)/GLUCOSE SYMPORTER
3	C, D, E (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
4	F (A)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 1 - 543 (UniProt: P96169)

PDB	External Database	Details
Ala 294	Lys 294	engineered mutation
Ala 411	Cys 411	engineered mutation
Cys 423	Ala 423	engineered mutation
Val 544	-	expression tag
Asn 545	-	expression tag
Ala 546	-	expression tag
Asp 547	-	expression tag
Ala 548	-	expression tag
Glu 549	-	expression tag
Ile 550	-	expression tag
Thr 551	-	expression tag
Leu 552	-	expression tag
Ile 553	-	expression tag
Ile 554	-	expression tag
Phe 555	-	expression tag
Gly 556	-	expression tag
Val 557	-	expression tag
Met 558	-	expression tag
Ala 559	-	expression tag
Gly 560	-	expression tag
Val 561	-	expression tag
Ile 562	-	expression tag
Gly 563	-	expression tag
Thr 564	-	expression tag
Ile 565	-	expression tag
Leu 566	-	expression tag
Leu 567	-	expression tag
Ile 568	-	expression tag
Ser 569	-	expression tag
Tyr 570	-	expression tag
Gly 571	-	expression tag
Ile 572	-	expression tag
Lys 573	-	expression tag
Lys 574	-	expression tag
Leu 575	-	expression tag
Ile 576	-	expression tag
Lys 577	-	expression tag
Ala 578	-	expression tag
Ser 579	-	expression tag
Tyr 580	-	expression tag
Lys 581	-	expression tag
Ser 582	-	expression tag
Gly 583	-	expression tag
Gly 584	-	expression tag
Ser 585	-	expression tag
Pro 586	-	expression tag
Gly 587	-	expression tag
His 588	-	expression tag
His 589	-	expression tag
His 590	-	expression tag
His 591	-	expression tag
His 592	-	expression tag
His 593	-	expression tag

B: 1 - 50 (PDB: 2XQ2)

PDB	External Database	Details
Val 544	-	expression tag
Asn 545	-	expression tag
Ala 546	-	expression tag
Asp 547	-	expression tag
Ala 548	-	expression tag
Glu 549	-	expression tag
Ile 550	-	expression tag
Thr 551	-	expression tag
Leu 552	-	expression tag
Ile 553	-	expression tag
Ile 554	-	expression tag
Phe 555	-	expression tag
Gly 556	-	expression tag
Val 557	-	expression tag
Met 558	-	expression tag
Ala 559	-	expression tag
Gly 560	-	expression tag
Val 561	-	expression tag
Ile 562	-	expression tag
Gly 563	-	expression tag
Thr 564	-	expression tag
Ile 565	-	expression tag
Leu 566	-	expression tag
Leu 567	-	expression tag
Ile 568	-	expression tag
Ser 569	-	expression tag
Tyr 570	-	expression tag
Gly 571	-	expression tag
Ile 572	-	expression tag
Lys 573	-	expression tag
Lys 574	-	expression tag
Leu 575	-	expression tag
Ile 576	-	expression tag
Lys 577	-	expression tag
Ala 578	-	expression tag
Ser 579	-	expression tag
Tyr 580	-	expression tag
Lys 581	-	expression tag
Ser 582	-	expression tag
Gly 583	-	expression tag
Gly 584	-	expression tag
Ser 585	-	expression tag
Pro 586	-	expression tag
Gly 587	-	expression tag
His 588	-	expression tag
His 589	-	expression tag
His 590	-	expression tag
His 591	-	expression tag
His 592	-	expression tag
His 593	-	expression tag

B: 51 - 543 (UniProt: P96169)

PDB	External Database	Details
Ala 294	Lys 294	engineered mutation
Ala 411	Cys 411	engineered mutation
Cys 423	Ala 423	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	127001.5
Non-Polymers*	Number of molecules	3
	Total formula weight	318.4
All*	Total formula weight	127319.9

*Water molecules are not included.