2XNJ
Crystal structure of an engineered Ferredoxin(flavodoxin) NADP(H) Reductase (FPR) from Escherichia coli
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | FERREDOXIN NADP-H REDUCTASE | polymer | 266 | 29547.0 | 2 | UniProt (P28861) Pfam (PF00970) Pfam (PF00175) In PDB | ESCHERICHIA COLI | |
| 2 | A, B | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 2 | Chemie (FAD) | |||
| 3 | A, B | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 2 | Chemie (NAP) | |||
| 4 | A, B | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 2 | Chemie (TRS) | |||
| 5 | A, B | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 6 | A, B | ZINC ION | non-polymer | 65.4 | 12 | Chemie (ZN) | |||
| 7 | B | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 8 | water | water | 18.0 | 410 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 256 (UniProt: P28861)
| PDB | External Database | Details |
|---|---|---|
| Gly -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Met -7 | - | expression tag |
| Ala -6 | - | expression tag |
| Ala -5 | - | expression tag |
| Ala -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Arg -2 | - | expression tag |
| Arg -1 | - | expression tag |
| Ile 0 | - | expression tag |
| Leu 1 | - | expression tag |
| Val 71 | Pro 71 | engineered mutation |
| Tyr 72 | Asp 72 | engineered mutation |
| Thr 73 | - | insertion |
| Asn 74 | - | insertion |
| Asp 75 | - | insertion |
| Ala 76 | - | insertion |
| Gly 77 | - | insertion |
| Glu 78 | - | insertion |
| Val 79 | - | insertion |
| Val 80 | - | insertion |
| Lys 81 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 59093.9 | |
| Non-Polymers* | Number of molecules | 22 |
| Total formula weight | 4386.4 | |
| All* | Total formula weight | 63480.3 |
