2XNJ

Crystal structure of an engineered Ferredoxin(flavodoxin) NADP(H) Reductase (FPR) from Escherichia coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	FERREDOXIN NADP-H REDUCTASE	polymer	266	29547.0	2	UniProt (P28861) Pfam (PF00970) Pfam (PF00175)	ESCHERICHIA COLI
2	C, M (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	D, N (A, B)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	2	Chemie (NAP)
4	E, O (A, B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
5	F, P, Q (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6	G, H, I, J, K... (A, B)	ZINC ION	non-polymer		65.4	12	Chemie (ZN)
7	X (B)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
8	Y, Z (A, B)	water	water		18.0	410	Chemie (HOH)

Sequence modifications

A, B: 2 - 256 (UniProt: P28861)

PDB	External Database	Details
Gly -9	-	expression tag
Ser -8	-	expression tag
Met -7	-	expression tag
Ala -6	-	expression tag
Ala -5	-	expression tag
Ala -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Arg -1	-	expression tag
Ile 0	-	expression tag
Leu 1	-	expression tag
Val 71	Pro 71	engineered mutation
Tyr 72	Asp 72	engineered mutation
Thr 73	-	insertion
Asn 74	-	insertion
Asp 75	-	insertion
Ala 76	-	insertion
Gly 77	-	insertion
Glu 78	-	insertion
Val 79	-	insertion
Val 80	-	insertion
Lys 81	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59093.9
Non-Polymers*	Number of molecules	22
	Total formula weight	4386.4
All*	Total formula weight	63480.3

*Water molecules are not included.