2XJF

Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II with a covalently modified Asn52

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CYTOSOLIC PURINE 5'-NUCLEOTIDASE	polymer	555	64132.0	1	UniProt (P49902) Pfam (PF05761)	HOMO SAPIENS (HUMAN)	CYTOSOLIC 5'-NUCLEOTIDASE II
2	B (A)	(2R)-2,3-diphosphoglyceric acid	non-polymer		266.0	1	Chemie (DG2)
3	C, D, E (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	F (A)	water	water		18.0	250	Chemie (HOH)

Sequence modifications

A: 1 - 536 (UniProt: P49902)

PDB	External Database	Details
Met -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
A5n 52	Asp 52	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	64132.0
Non-Polymers*	Number of molecules	4
	Total formula weight	542.3
All*	Total formula weight	64674.3

*Water molecules are not included.