2XJF
Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II with a covalently modified Asn52
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.095, 128.222, 130.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.230 - 2.100 |
| R-factor | 0.18084 |
| Rwork | 0.178 |
| R-free | 0.22517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jc9 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.625 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.440 |
| Number of reflections | 43556 | |
| <I/σ(I)> | 11.7 | 2.9 |
| Completeness [%] | 96.4 | 98.1 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BICINE PH 9.0, 10% PEG 6000. |
