2XH2

Engineering the enolase active site pocket: Crystal structure of the S39N D321A mutant of yeast enolase 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	ENOLASE 1	polymer	443	47671.9	4	UniProt (P00924) Pfam (PF03952) Pfam (PF00113)	SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)	2-PHOSPHOGLYCERATE DEHYDRATASE 1,2-PHOSPHO-D-GLYCERATE HYDRO-LYASE 1
2	E, G, I, K (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	F, H, J, L (A, B, C, D)	2-PHOSPHOGLYCERIC ACID	non-polymer		186.1	4	Chemie (2PG)
4	M, N, O, P (A, B, C, D)	water	water		18.0	2567	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 436 (UniProt: P00924)

PDB	External Database	Details
Leu 437	-	expression tag
His 438	-	expression tag
His 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
His 442	-	expression tag
His 443	-	expression tag
Asn 39	Ser 40	engineered mutation
Ile 241	Val 242	SEE REMARK 999
Ala 321	Asp 322	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	190687.5
Non-Polymers*	Number of molecules	8
	Total formula weight	841.4
All*	Total formula weight	191528.9

*Water molecules are not included.