2XH2
Engineering the enolase active site pocket: Crystal structure of the S39N D321A mutant of yeast enolase 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-10-08 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 64.720, 82.540, 95.610 |
Unit cell angles | 89.41, 71.31, 84.80 |
Refinement procedure
Resolution | 35.650 - 1.800 |
R-factor | 0.16797 |
Rwork | 0.166 |
R-free | 0.20949 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xh0 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.181 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.900 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.130 | 0.490 |
Number of reflections | 162601 | |
<I/σ(I)> | 12.74 | 3.48 |
Completeness [%] | 93.1 | 87.3 |
Redundancy | 2.01 | 1.77 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 18 % PEG 3350, 0.2 M POTASSIUM SULFATE |