2XH0

Engineering the enolase active site pocket: Crystal structure of the S39N Q167K D321R mutant of yeast enolase 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	ENOLASE 1	polymer	443	47759.0	4	UniProt (P00924) Pfam (PF03952) Pfam (PF00113) In PDB	SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)	2-PHOSPHOGLYCERATE DEHYDRATASE 1,2-PHOSPHO-D-GLYCERATE HYDRO-LYASE 1
2	A, B, C, D	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	A, B, C, D	PHOSPHOENOLPYRUVATE	non-polymer		168.0	4	Chemie (PEP)
4		water	water		18.0	1569	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 436 (UniProt: P00924)

PDB	External Database	Details
Leu 437	-	expression tag
His 438	-	expression tag
His 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
His 442	-	expression tag
His 443	-	expression tag
Asn 39	Ser 40	engineered mutation
Lys 167	Gln 168	engineered mutation
Ile 241	Val 242	conflict
Arg 321	Asp 322	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	191036.1
Non-Polymers*	Number of molecules	8
	Total formula weight	769.4
All*	Total formula weight	191805.5

*Water molecules are not included.