2XH0
Engineering the enolase active site pocket: Crystal structure of the S39N Q167K D321R mutant of yeast enolase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-09-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.329, 60.797, 120.667 |
| Unit cell angles | 89.89, 89.90, 65.84 |
Refinement procedure
| Resolution | 37.850 - 1.700 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2one |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.315 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.160 | 0.770 |
| Number of reflections | 147095 | |
| <I/σ(I)> | 9.69 | 2.34 |
| Completeness [%] | 93.0 | 86.9 |
| Redundancy | 1.97 | 1.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 25% PEG 4000, 0.1M SODIUM ACETATE PH 4.6 |
