2XGZ
Engineering the enolase active site pocket: Crystal structure of the S39N D321R mutant of yeast enolase 1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | ENOLASE 1 | polymer | 443 | 47758.0 | 2 | UniProt (P00924) Pfam (PF03952) Pfam (PF00113) | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | 2-PHOSPHOGLYCERATE DEHYDRATASE, 1,2-PHOSPHO-D-GLYCERATE HYDRO-LYASE 1 |
| 2 | C, E, F, G, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 3 | D, H (A, B) | PHOSPHOENOLPYRUVATE | non-polymer | 168.0 | 2 | Chemie (PEP) | |||
| 4 | J, K (A, B) | water | water | 18.0 | 799 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 436 (UniProt: P00924)
| PDB | External Database | Details |
|---|---|---|
| Leu 437 | - | expression tag |
| His 438 | - | expression tag |
| His 439 | - | expression tag |
| His 440 | - | expression tag |
| His 441 | - | expression tag |
| His 442 | - | expression tag |
| His 443 | - | expression tag |
| Asn 39 | Ser 40 | engineered mutation |
| Ile 241 | Val 242 | conflict |
| Arg 321 | Asp 322 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 95516.0 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 457.6 | |
| All* | Total formula weight | 95973.6 |
