2XGZ

Engineering the enolase active site pocket: Crystal structure of the S39N D321R mutant of yeast enolase 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ENOLASE 1	polymer	443	47758.0	2	UniProt (P00924) Pfam (PF03952) Pfam (PF00113) In PDB	SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)	2-PHOSPHOGLYCERATE DEHYDRATASE, 1,2-PHOSPHO-D-GLYCERATE HYDRO-LYASE 1
2	A, B	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
3	A, B	PHOSPHOENOLPYRUVATE	non-polymer		168.0	2	Chemie (PEP)
4		water	water		18.0	799	Chemie (HOH)

Sequence modifications

A, B: 1 - 436 (UniProt: P00924)

PDB	External Database	Details
Leu 437	-	expression tag
His 438	-	expression tag
His 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
His 442	-	expression tag
His 443	-	expression tag
Asn 39	Ser 40	engineered mutation
Ile 241	Val 242	conflict
Arg 321	Asp 322	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95516.0
Non-Polymers*	Number of molecules	7
	Total formula weight	457.6
All*	Total formula weight	95973.6

*Water molecules are not included.