2XGZ
Engineering the enolase active site pocket: Crystal structure of the S39N D321R mutant of yeast enolase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-09-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.380, 63.050, 64.430 |
| Unit cell angles | 73.01, 79.28, 81.22 |
Refinement procedure
| Resolution | 37.900 - 1.800 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2one |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.245 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.160 | 0.900 |
| Number of reflections | 64875 | |
| <I/σ(I)> | 11.66 | 2.05 |
| Completeness [%] | 75.9 | 76.8 |
| Redundancy | 1.65 | 1.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% PEG 8000, 0.2M SODIUM ACETATE, 0.1M SODIUM CACODYLATE PH 6.5 |
