2XFW

Structure of the E192N mutant of E. coli N-acetylneuraminic acid lyase in complex with pyruvate in crystal form III

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	N-ACETYLNEURAMINIC ACID LYASE	polymer	304	33638.4	4	UniProt (B7M0T7) Pfam (PF00701) UniProt (by SIFTS) (P0A6L4)	ESCHERICHIA COLI	N-ACETYLNEURAMINIC ACID ALDOLASE, N-ACETYLNEURAMINATE PYRUVATE-LYASE, SIALIC ACID LYASE, SIALATE LYASE, SIALIC ACID ALDOLASE
2	E, H, I, J, K... (A, B, C, D)	PYRUVIC ACID	non-polymer		88.1	6	Chemie (PYR)
3	F, G, M, N (A, D)	PENTAETHYLENE GLYCOL	non-polymer		238.3	4	Chemie (1PE)
4	O, P, Q, R (A, B, C, D)	water	water		18.0	977	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 297 (UniProt: B7M0T7)

PDB	External Database	Details
Met -6	-	expression tag
Glu -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Asn 192	Glu 192	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	134553.6
Non-Polymers*	Number of molecules	10
	Total formula weight	1481.5
All*	Total formula weight	136035.1

*Water molecules are not included.