2XED

Nocardia farcinica maleate cis-trans isomerase C194S mutant with a covalently bound succinylcysteine intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	PUTATIVE MALEATE ISOMERASE	polymer	273	28827.0	4	UniProt (Q5YXQ1) Pfam (PF17645)	NOCARDIA FARCINICA
2	E, F, G, H (A, B, C, D)	SUCCINIC ACID	non-polymer		118.1	4	Chemie (SIN)
3	I, J, K, L (A, B, C, D)	water	water		18.0	635	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 251 (UniProt: Q5YXQ1)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Ala 0	-	expression tag
Ala 194	Cys 194	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	115307.9
Non-Polymers*	Number of molecules	4
	Total formula weight	472.4
All*	Total formula weight	115780.3

*Water molecules are not included.