2XED

Nocardia farcinica maleate cis-trans isomerase C194S mutant with a covalently bound succinylcysteine intermediate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016853	molecular_function	isomerase activity
A	0050076	molecular_function	maleate isomerase activity
B	0016853	molecular_function	isomerase activity
B	0050076	molecular_function	maleate isomerase activity
C	0016853	molecular_function	isomerase activity
C	0050076	molecular_function	maleate isomerase activity
D	0016853	molecular_function	isomerase activity
D	0050076	molecular_function	maleate isomerase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SIN A 300

Chain	Residue
A	PRO11
A	GLN196
A	ASN14
A	CYS76
A	LEU77
A	VAL78
A	TYR133
A	ASN163
A	ALA194
A	VAL195

---

site_id	AC2
Number of Residues	11
Details	BINDING SITE FOR RESIDUE SIN B 300

Chain	Residue
B	PRO11
B	ASN14
B	CYS76
B	LEU77
B	VAL78
B	TYR133
B	ASN163
B	ALA194
B	VAL195
B	GLN196
B	HOH2102

---

site_id	AC3
Number of Residues	11
Details	BINDING SITE FOR RESIDUE SIN C 300

Chain	Residue
C	PRO11
C	ASN14
C	MET42
C	CYS76
C	LEU77
C	VAL78
C	TYR133
C	ASN163
C	ALA194
C	VAL195
C	GLN196

---

site_id	AC4
Number of Residues	11
Details	BINDING SITE FOR RESIDUE SIN D 300

Chain	Residue
D	PRO11
D	ASN14
D	CYS76
D	LEU77
D	VAL78
D	TYR133
D	ASN163
D	ALA194
D	VAL195
D	GLN196
D	HOH2099

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:20677745

Chain	Residue	Details
A	CYS76
B	CYS76
C	CYS76
D	CYS76

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:20677745

Chain	Residue	Details
A	ALA194
B	ALA194
C	ALA194
D	ALA194

---

site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING: BINDING => ECO:0000269|PubMed:20677745

Chain	Residue	Details
A	ASN14
B	VAL195
C	ASN14
C	CYS76
C	TYR133
C	ASN163
C	VAL195
D	ASN14
D	CYS76
D	TYR133
D	ASN163
A	CYS76
D	VAL195
A	TYR133
A	ASN163
A	VAL195
B	ASN14
B	CYS76
B	TYR133
B	ASN163

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: S-(2-succinyl)cysteine => ECO:0000269|PubMed:20677745, ECO:0007744|PDB:2XED

Chain	Residue	Details
A	CYS76
B	CYS76
C	CYS76
D	CYS76

---