2XE0

Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	I-CREI V2V3 VARIANT	polymer	152	17068.6	1		CHLAMYDOMONAS REINHARDTII
2	B (B)	I-CREI V2V3 VARIANT	polymer	152	16999.4	1		CHLAMYDOMONAS REINHARDTII
3	C (C)	24MER DNA	polymer	24	7320.7	1		HOMO SAPIENS	5'-D(*TP*TP*GP*TP*TP*CP*TP*CP*AP*GP*GP*TP *AP*CP*CP*TP*CP*AP*GP*CP*CP*AP*GP*A)-3'
4	D (D)	24MER DNA	polymer	24	7418.8	1		HOMO SAPIENS	5'-D(*TP*CP*TP*GP*GP*CP*TP*GP*AP*GP*GP*TP*AP*CP CP*TP*GP*AP*GP*AP*AP*CP*AP*A)-3'
5	E, F, G, I (A, B)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
6	H (B)	S-1,2-PROPANEDIOL	non-polymer		76.1	1	Chemie (PGO)
7	J, K, L (C, D)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
8	M, N, O, P (A, B, C, D)	water	water		18.0	63	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	48807.5
Non-Polymers*	Number of molecules	8
	Total formula weight	385.2
All*	Total formula weight	49192.7

*Water molecules are not included.