2XE0

Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS
Synchrotron site	SLS
Temperature [K]	100
Spacegroup name	P 21 21 2
Unit cell lengths	177.857, 71.538, 45.005
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.769 - 2.310
R-factor	0.2152
Rwork	0.213
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g9z
RMSD bond length	0.008
RMSD bond angle	1.309
Data reduction software	XDS
Data scaling software	XSCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.760	35.760
High resolution limit [Å]	2.310	2.400
Rmerge	0.440	0.060
Number of reflections	24763
<I/σ(I)>	1.1	12.1
Completeness [%]	74.4	94.9
Redundancy	2.7	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1