2X8S
Crystal Structure of the Abn2 D171A mutant in complex with arabinotriose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | ENDO-ALPHA-1,5-L-ARABINANASE | polymer | 470 | 52738.1 | 2 | UniProt (B3FRL6) Pfam (PF04616) Pfam (PF16369) UniProt (by SIFTS) (P42293) | BACILLUS SUBTILIS | ENDO-ALPHA- ARABINANASE |
| 2 | C, D (C, D) | alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose | branched | 414.4 | 2 | In PDB GlyTouCan (G56809YT) | |||
| 3 | E, K, N (A, B) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 4 | F, G, H, L, P... (A, B) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 5 | I (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 6 | J (B) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 7 | M, Q (B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 8 | O (B) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 9 | S (B) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 1 | Chemie (MPD) | |||
| 10 | T, U (A, B) | water | water | 18.0 | 691 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 469 (UniProt: B3FRL6)
| PDB | External Database | Details |
|---|---|---|
| Ala 171 | Asp 171 | engineered mutation |
| Leu 470 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 105476.2 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 828.7 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 1080.3 | |
| All* | Total formula weight | 107385.2 |
