2X8S

Crystal Structure of the Abn2 D171A mutant in complex with arabinotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	110
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	51.950, 57.985, 85.595
Unit cell angles	96.14, 91.77, 117.34

Refinement procedure

Resolution	24.595 - 1.500
R-factor	0.1482
Rwork	0.147
R-free	0.16660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x8f
RMSD bond length	0.017
RMSD bond angle	1.403
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.800	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.040	0.380
Number of reflections	133640
Completeness [%]	94.9	92.7
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1