2X8S
Crystal Structure of the Abn2 D171A mutant in complex with arabinotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 110 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 51.950, 57.985, 85.595 |
Unit cell angles | 96.14, 91.77, 117.34 |
Refinement procedure
Resolution | 24.595 - 1.500 |
R-factor | 0.1482 |
Rwork | 0.147 |
R-free | 0.16660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x8f |
RMSD bond length | 0.017 |
RMSD bond angle | 1.403 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.800 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.040 | 0.380 |
Number of reflections | 133640 | |
Completeness [%] | 94.9 | 92.7 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |