2X8J

Intracellular subtilisin precursor from B. clausii

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D, E, F	INTRACELLULAR SUBTILISIN PROTEASE	polymer	327	34717.7	5	UniProt (D0AB41) Pfam (PF00082) In PDB	BACILLUS CLAUSII	INTRACELLULAR SUBTILISIN
2	B	INTRACELLULAR SUBTILISIN PROTEASE	polymer	327	34733.7	1	UniProt (D0AB41) Pfam (PF00082) In PDB	BACILLUS CLAUSII	INTRACELLULAR SUBTILISIN
3	A, B, C, D, E...	SODIUM ION	non-polymer		23.0	6	Chemie (NA)
4	A, B, C, D, E...	PENTAETHYLENE GLYCOL	non-polymer		238.3	6	Chemie (1PE)
5	C, D	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	1501	Chemie (HOH)

Sequence modifications

A, C, D, E, F: 1 - 321 (UniProt: D0AB41)

PDB	External Database	Details
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
Ala 250	Ser 250	engineered mutation

B: 1 - 321 (UniProt: D0AB41)

PDB	External Database	Details
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
Ala 250	Ser 250	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	208322.5
Non-Polymers*	Number of molecules	14
	Total formula weight	1751.8
All*	Total formula weight	210074.3

*Water molecules are not included.